![]() ![]() Recently, a more elegant variation of extraction device prefilled with method-specific extraction buffer and sampling pin has been introduced. The first version of such device, Smart-Prep faecal sample preparation kit (Roche diagnostics, Manheim, Germany), might be used for all methods, has a sample chamber carrying approximately 85 mg of stool sample and 4 mL of method specific extraction buffer is added to obtain 1:50 dilution ( 13, 14). Faecal sample weighing, as the gold standard FC extraction method, is rather impractical and time-consuming thus extraction devices have been introduced as convenient alternative ( 12). The lack of overall process standardization, from faecal sample preparation to methodology used for FC measurement, results in great variability between commercially available assays ( 7- 11). As the most widely used faecal biomarker, FC has been recommended for diagnosis and differentiation between inflammatory bowel disease (IBD) and irritable bowel syndrome in adults and paediatric population, for monitoring treatment response, mucosal healing process or predicting disease relapse ( 3- 6). Its determination substantially reduces the need for invasive endoscopy with biopsy which is expensive but nevertheless regarded as gold standard for assessing mucosal inflammation ( 1, 2). ![]() Faecal calprotectin (FC) is a stable, feasible biomarker, which is released in stool through neutrophils´ disruption during inflammation in bowel mucosa.
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